U-Blot® RNA pol II Monoclonal Antibody
UcallM
$999.00
Product details
Background:RNA pol II ( RNA polymerase II ) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II , in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination.
Specifications
| Target | -- |
| Reactivity | Human, Mouse |
| Applications | ChIP; WB; IP |
| MW(Calculated) | 240 kDa |
| Host Species | Rat |
| Isotype | IgG1 |
| Recommended Dilution Ratio | For RNA Pol II , we also offer an AbFlex ® RNA Pol II Recombinant Antibody (rAb). For details, see Catalog No. 91151 . |
| Formulation | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 4F8 |
| Immunogen | This RNA pol II antibody was raised against a synthetic peptide corresponding to human RNA pol II C-terminal heptad repeat domain. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | RPB1, RPO2, POLR2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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