Name: U-Blot® SLP-76 Rabbit mAb
SKU: WR5174
Price: 268 USD
Availability: InStock

U-Blot® SLP-76 Rabbit mAb

Ucallm_WR5174-50

Price range: $268.00 through $328.00

Size

100μl

50μl

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SKU: WR5174-100

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Product details

Background:SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T c

Specifications

Size	50μL/100μL
Target	SLP-76
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	60kD
MW(Observed)	70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-1:200; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1408R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	LCP2
Protein Name	Lymphocyte cytosolic protein 2
Other Name	LCP2; / Lymphocyte cytosolic protein 2; / SH2 domain-containing leukocyte protein of 76 kDa; / SLP-76 tyrosine phosphoprotein; / SLP76
Species	Human
Gene ID	3937
Uniprot	Q13094,
Species.1	Mouse
Gene ID.1	16822
Uniprot.1	Q60787
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm .
Tissue Expression	Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Signaling_pathway	Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway
Research Areas	>>Rap1 signaling pathway; / >>Osteoclast differentiation; / >>Platelet activation; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Yersinia infection
Function	Domain:The SH2 domain mediates interaction with SHB.,Function:Involved in T-cell antigen receptor mediated signaling.,PTM:Phosphorylated after T-cell receptor activation by ZAP-70.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 1 SH2 domain.,subunit:Interacts with SLA. Interacts with CBLB (By similarity). Interacts with the adapter proteins GRB2 and FYB. Interacts with SHB. Interacts with PRAM1.,tissue specificity:Highly expressed in spleen, thymus, and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblasatoma cell lines.,