Name: U-Blot® SOCS-1 Rabbit mAb
SKU: WR5388
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	SOCS-1
Reactivity	Human
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	24kD
MW(Observed)	22kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:400; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1632R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	SOCS1
Protein Name	Suppressor of cytokine signaling 1
Other Name	SOCS1; / SSI1; / TIP3; / Suppressor of cytokine signaling 1; / SOCS-1; / JAK-binding protein; / JAB; / STAT-induced STAT inhibitor 1; / SSI-1; / Tec-interacting protein 3; / TIP-3
Species	Human
Gene ID	8651
Uniprot	O15524,
Species.1	Mouse
Gene ID.1	12703
Uniprot.1	O35716,
Species.2	Rat
Gene ID.2	252971
Uniprot.2	Q9QX78
Cellular Localization	Nucleus . Cytoplasmic vesicle . Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.
Tissue Expression	Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Research Areas	>>Ubiquitin mediated proteolysis; / >>Osteoclast differentiation; / >>JAK-STAT signaling pathway; / >>Insulin signaling pathway; / >>Prolactin signaling pathway; / >>Type II diabetes mellitus; / >>Growth hormone synthesis, secretion and action; / >>Toxoplasmosis; / >>MicroRNAs in cancer
Function	Domain:The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.,Domain:The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.,Function:SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.,induction:By a subset of cytokines including those belonging to the interferon, interleukin and colony-stimulating factor families.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SOCS box domain.,subunit:Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV (By similarity). Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC(SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1 (By similarity). Interacts with TRIM8 (By similarity). Interacts with CUL2.,tissue specificity:Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.,

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U-Blot® SOCS-1 Rabbit mAb

Product details

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U-Blot® SOCS-1 Rabbit mAb

Product details

Specifications

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