SKU: WR5361 Categories: ,

U-Blot® SPARC Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5361-50
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Product details

Background:This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],

Specifications

Size50μL/100μL
TargetSPARC
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)35kD
MW(Observed)39kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:500; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1604R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameSPARC
Protein NameSPARC
Other NameSPARC; / ON; / SPARC; / Basement-membrane protein 40; / BM-40; / Osteonectin; / ON; / Secreted protein acidic and rich in cysteine
SpeciesHuman
Gene ID6678
UniprotP09486,
Species.1Mouse
Gene ID.120692
Uniprot.1P07214,
Species.2Rat
Gene ID.224791
Uniprot.2P16975
Cellular LocalizationSecreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.
Tissue ExpressionMuscle,Placenta,PNS,Prostate,Skin,
Signaling_pathway
Research Areas
Functiondevelopmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,Function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane.,
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