SKU: WR5361 Categories: ,

U-Blot® SPARC Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5361-50
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Product details

Background:This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],

Specifications

TargetSPARC
ReactivityHuman,Mouse
ApplicationsWB,IHC,IF,ELISA
MW(Calculated)35kD
MW(Observed)39kD
Host SpeciesRabbit
IsotypeIgG,Kappa
ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:500;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
PurificationProtein A
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
ClonalityMonoclonal
Clone NumberPT1604R
SpecificityEndogenous
Gene NameSPARC
Protein NameSPARC
Other NameSPARC;ON;SPARC;Basement-membrane protein 40;BM-40;Osteonectin;ON;Secreted protein acidic and rich in cysteine
Organism-1Human
Gene ID-16678
SwissProt-1P09486
Organism-2Mouse
Gene ID-220692
SwissProt-2P07214
Organism-3Rat
Gene ID-324791
SwissProt-3P16975
Functiondevelopmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,Function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane.,
Cellular LocalizationSecreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane.
Tissue ExpressionMuscle,Placenta,PNS,Prostate,Skin,
Unnamed: 31
SwissProt-3.1
Organism-4
Gene ID-4
SwissProt-4
Cellular Localization.1
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