U-Blot® SPARC Rabbit mAb
Ucallm_WR5361-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015],
Specifications
| Size | 50μL/100μL |
| Target | SPARC |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 35kD |
| MW(Observed) | 39kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1604R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SPARC |
| Protein Name | SPARC |
| Other Name | SPARC; / ON; / SPARC; / Basement-membrane protein 40; / BM-40; / Osteonectin; / ON; / Secreted protein acidic and rich in cysteine |
| Species | Human |
| Gene ID | 6678 |
| Uniprot | P09486, |
| Species.1 | Mouse |
| Gene ID.1 | 20692 |
| Uniprot.1 | P07214, |
| Species.2 | Rat |
| Gene ID.2 | 24791 |
| Uniprot.2 | P16975 |
| Cellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane. |
| Tissue Expression | Muscle,Placenta,PNS,Prostate,Skin, |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,Function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane., |
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