U-Blot® SRF Monoclonal Antibody
UcallM
$999.00
Product details
Background:Tet1 (Ten-Eleven Translocation-1; methylcytosine dioxygenase) is a protein that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) which can lead to cytosine demethylation by either further oxidation via Tet proteins to 5-formylcytosine (5fC) and 5-carboxycytosine (5caC) or deamination into 5-hydroxymethyluracil (5hmU) and then subsequent replacement by unmethylated cytosine by the base excision repair system. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. Tet1 preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and polycomb-repressed genes. By controlling the levels of 5mC and 5hmC at gene promoters, it may regulate the gene expression silencing induced by cytosine methylation. May have a dual function by also repressing the expression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells, it plays a role in embryonic stem cells maintenance and inner cell mass cell specification.
Specifications
| Target | -- |
| Reactivity | Human, Mouse |
| Applications | ChIP; ChIP-Seq; WB; IF; IHC |
| MW(Calculated) | 67 kDa |
| Host Species | Rat |
| Isotype | IgG2a |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP: 5 - 10 µl per ChIP ChIP-Seq: 5 - 10 µl each WB: 0.5 - 2 µg/ml ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here . |
| Formulation | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 2C5 |
| Immunogen | This antibody was raised against a full-length protein corresponding to mouse SRF. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | -- |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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