Specifications
| Target | STAT3 |
| Reactivity | Human |
| Applications | ICFC,ICC, ChIP, |
| MW(Calculated) | -- |
| Host Species | Mouse |
| Isotype | Mouse IgG1, κ |
| Recommended Dilution Ratio | Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol . For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration of 2.5 μg/mL is recommended. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Purification | The antibody was purified by affinity chromatography. |
| Storage | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Concentration | 0.5 mg/ml |
| Clonality | Monoclonal |
| Clone Number | RMM6089 |
| Immunogen | Human Stat3 peptide phosphorylated at Ser 727. Complete Freund's adjuvant. |
| Specificity | STAT3 Phospho Ser727 |
| Gene Name | -- |
| Other Name | Signal transducer and activator of transcription 3, Acute-phase response factor (APRF), HIES, ADMIO |
| Gene ID | 6774 |
| Cellular Localization | Nuclear Markers, Phospho-Proteins |
| Function | STAT3 is also involved in gp130 signaling and binds to IL-6 response elements in various acute phase protein promoters. STAT3 is phosphorylated by signaling from IFNs, EGF, FGF, IL-5, HGF, LIF, and BMP2. STAT3 activity is inhibited by PIAS3 and GRIM-19 and can also be regulated by the Rac1 protein. |
