U-Blot® Suz12 Monoclonal Antibody
UcallM
$999.00
Product details
Background:AbFlex ® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex ® TWIST antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. TWIST is a regulator of transcription that serves as a master regulator of embryonic morphogenesis. Through its bHLH motif it interacts with many different transcription factors to affect multiple pathways of gene expression. TWIST plays a pivotal role in mesodermal, myoblast, and osteoblast differentiation. TWIST is involved in the epithelial–mesenchymal transition (EMT), a period during embryonic development characterized by loss of cell adhesion and increased cell mobility, essential events for mesoderm and neural tube formation. The EMT shares many features of metastatic cancer, and elevated levels of TWIST are observed in many types of cancer. Mutations in the gene encoding TWIST have been associated with Saethre-Chotzen syndrome, an inherited developmental disorder characterized by skull deformations, limb abnormalities, and facial dysmorphisim.
Specifications
| Target | -- |
| Reactivity | Human |
| Applications | ChIP; WB |
| MW(Calculated) | 95 kDa |
| Host Species | Mouse |
| Isotype | IgG2b |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP: 2.5 - 10 µg per ChIP This antibody has been published, but has not been validated by Active Motif, for Western blot. For an antibody validated for use in Western blotting, see Catalog No. 39357 . This antibody is also available as an AbFlex ® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91163 . |
| Formulation | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 2AO9 |
| Immunogen | This Suz12 antibody was raised against a recombinant protein corresponding to full-length human Suz12. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | CHET9, JJAZ1 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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