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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® TFE3 Rabbit mAb
SKU: WR5268 Categories: ,

U-Blot® TFE3 Rabbit mAb

Ucallm_WR5268-50

Price range: $268.00 through $328.00

SKU: WR5268-100
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Product details

Background:This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013],

Specifications

Size50μL/100μL
TargetTFE3
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)62kD
MW(Observed)72-89kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:100-1:500; WB 1:1000-1:20000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1512R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameTFE3 BHLHE33
Protein NameTranscription factor E3
Other NameTFE3; / BHLHE33; / Transcription factor E3; / Class E basic helix-loop-helix protein 33; / bHLHe33
SpeciesHuman
Gene ID7030
UniprotP19532,
Species.1Mouse
Gene ID.1209446
Uniprot.1Q64092
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm, cytosol . Nucleus . When nutrients are present, phosphorylation by MTOR prevents nuclear translocation and activity (PubMed:22692423, PubMed:30733432). Conversely, inhibition of mTORC1, starvation and lysosomal disruption, promotes dephosphorylation and translocation to the nucleus (PubMed:22692423, PubMed:30733432). .
Tissue ExpressionUbiquitous in fetal and adult tissues.
Signaling_pathwayCellular Processes >> Transport and catabolism >> Mitophagy - animal;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Renal cell carcinoma
Research Areas>>Mitophagy - animal; / >>Transcriptional misregulation in cancer; / >>Renal cell carcinoma
FunctionDisease:Chromosomal aberrations involving TFE3 are recurrent in alveolar soft part sarcoma (ASPS) [MIM:606243]. Translocation t(X; 17)(p11; q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.,Disease:Chromosomal aberrations involving TFE3 are recurrent in alveolar soft part sarcoma (ASPS). Translocation t(X; 17)(p11; q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.,Disease:Chromosomal aberrations involving TFE3 may be a cause of papillary renal cell carcinoma (PRCC) [MIM:605074]. Translocation t(X; 1)(p11.2; q21.2) with PRCC; translocation t(X; 1)(p11.2; p34) with PSF; inversion inv(X)(p11.2; q12) that fuses NONO to TFE3.,Function:Positive-acting transcription factor that binds to the immunoglobulin enchancer MUE3 motif. It also binds very well to a USF/MLTF site. Binding of TFE3 to DNA induces DNA binding.,similarity:Belongs to the MiT/TFE family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,tissue specificity:Ubiquitous in fetal and adult tissues.,
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