U-Blot® TGF-α Rabbit mAb
Ucallm_WR5896-50
Price range: $268.00 through $328.00
Product details
Background：
This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor , which activates a signaling pathway for cell proliferation , differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers , and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Sep 2011] ,
Specifications
| Size | 50μL/100μL |
| Target | TGFα |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 17kD |
| MW(Observed) | 19kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1724R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | TGFA |
| Protein Name | Protransforming growth factor alpha |
| Other Name | TGFA; / Protransforming growth factor alpha |
| Species | Human |
| Gene ID | 7039 |
| Uniprot | P01135, |
| Species.1 | Mouse |
| Gene ID.1 | 21802 |
| Uniprot.1 | P48030, |
| Species.2 | Rat |
| Gene ID.2 | 24827 |
| Uniprot.2 | P01134 |
| Cellular Localization | [Transforming growth factor alpha]: Secreted, extracellular space.; [Protransforming growth factor alpha]: Cell membrane; Single-pass type I membrane protein. |
| Tissue Expression | Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>PI3K-Akt signaling pathway; / >>Estrogen signaling pathway; / >>Pathways in cancer; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Glioma; / >>Prostate cancer; / >>Non-small cell lung cancer; / >>Hepatocellular carcinoma |
| Function | Function:TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.,similarity:Contains 1 EGF-like domain.,subunit:Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2., |
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