Name: U-Blot® UBC12 Rabbit mAb
SKU: WR5381
Price: 268 USD
Availability: InStock

U-Blot® UBC12 Rabbit mAb

Ucallm_WR5381-50

Price range: $268.00 through $328.00

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50μl

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SKU: WR5381-50

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Product details

Background:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	UBC12
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	21kD
MW(Observed)	21kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1625R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	UBE2M UBC12
Protein Name	UBC12
Other Name
Species	Human
Gene ID	9040
Uniprot	P61081,
Species.1	Mouse
Gene ID.1	22192
Uniprot.1	P61082
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	cytoplasm,cytosol,extracellular exosome,
Tissue Expression
Signaling_pathway	Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis
Research Areas	>>Ubiquitin mediated proteolysis
Function	Catalytic activity:ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.,Domain:Both the N-terminal docking peptide and the catalytic core domain must bind the uba3-nae1 complex simultaneously for optimal transfer of nedd8.,Function:Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase rbx1, but not rbx2, suggests that the rbx1-ube2m complex neddylates specific target proteins, such as cul1, cul2, cul3 and cul4. Involved in cell proliferation.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.,subunit:Interacts with UBA3 and RBX1.,