U-Blot® VPS4A Rabbit mAb
Ucallm_WR6082-50
Price range: $268.00 through $328.00
Product details
Background：
Specifications
| Size | 50μL/100μL |
| Target | VPS4A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 49kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1993R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | VPS4A VPS4 |
| Protein Name | Vacuolar protein sorting-associated protein 4A (Protein SKD2) (VPS4-1) (hVPS4) |
| Other Name | |
| Species | Human |
| Gene ID | 27183 |
| Uniprot | Q9UN37, |
| Species.1 | Mouse |
| Gene ID.1 | 116733 |
| Uniprot.1 | Q8VEJ9, |
| Species.2 | Rat |
| Gene ID.2 | 246772 |
| Uniprot.2 | Q793F9 |
| Cellular Localization | Late endosome membrane; Peripheral membrane protein . Midbody . Cytoplasm , cytoskeleton , spindle . Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells , interaction with ZFYVE19/ANCHR mediates retention at midbody (PubMed:24814515) . Localized in two distinct rings on either side of the Flemming body. . |
| Tissue Expression | Ubiquitously expressed. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Necroptosis |
| Research Areas | |
| Function | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly , possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins , such as stimulated growth factor receptors , lysosomal enzymes and lipids. It is required for proper accomplishment of various processes including the regulation of endosome size , primary cilium organization , mitotic spindle organization , chromosome segregation , and nuclear envelope sealing and spindle disassembly during anaphase . Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C , leading to abscission . VPS4A/B are required for the exosomal release of SDCBP , CD63 and syndecan . Critical for normal erythroblast cytokinesis and correct erythropoiesis .; (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events , such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses) . |
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