UBA3 rabbit pAb
Ucallm_WP16762-50
Price range: $268.00 through $328.00
Product details
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | UBA3 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 51kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human UBA3 AA range: 105-155 |
| Specificity | This antibody detects endogenous levels of UBA3 at Human/Mouse/Rat |
| Gene Name | UBA3 UBE1C |
| Protein Name | UBA3 |
| Other Name | |
| Species | Human |
| Gene ID | 9039 |
| Uniprot | Q8TBC4, |
| Species.1 | Mouse |
| Gene ID.1 | 22200 |
| Uniprot.1 | Q8C878, |
| Species.2 | Rat |
| Gene ID.2 | 117553 |
| Uniprot.2 | Q99MI7 |
| Cellular Localization | nucleus,cytosol, |
| Tissue Expression | Ubiquitously expressed. |
| Signaling_pathway | Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis |
| Function | enzyme regulation:Binding of TP53BP2 to the regulatory subunit NAE1 decreases activity.,Function:Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.,miscellaneous:Arg-211 acts as a selectivity gate, preventing misactivation of ubiquitin by this NEDD8-specific E1 complex.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.,subunit:Heterodimer of UBA3 and NAE1. Interacts with NEDD8, UBE2F and UBE2M. Binds ESR1 and ESR2 with bound steroid ligand.,tissue specificity:Ubiquitously expressed., |
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