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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Mouse mAb/UBC9 Monoclonal Antibody
SKU: WM0459 Categories: ,

UBC9 Monoclonal Antibody

Ucallm_WM0459-50

Price range: $268.00 through $328.00

SKU: WM0459-50
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Product details

Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetUBC9
ReactivityHuman
ApplicationsWB, IHC, IF, FC, ELISA
MW(Calculated)18kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number10A8
ImmunogenPurified recombinant fragment of human UBC9 expressed in E. Coli.
SpecificityUBC9 Monoclonal Antibody detects endogenous levels of UBC9 protein.
Gene NameUBE2I UBC9 UBCE9
Protein NameSUMO-conjugating enzyme UBC9
Other NameUBE2I; / UBC9; / UBCE9; / SUMO-conjugating enzyme UBC9; / SUMO-protein ligase; / Ubiquitin carrier protein 9; / Ubiquitin carrier protein I; / Ubiquitin-conjugating enzyme E2 I; / Ubiquitin-protein ligase I; / p18
SpeciesHuman
Gene ID7329
UniprotP63279
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Cytoplasm . Cytoplasm, perinuclear region . Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). .
Tissue ExpressionExpressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Signaling_pathwayHuman Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Genetic Information Processing >> Translation >> Nucleocytoplasmic transport;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis
Research Areas>>Nucleocytoplasmic transport; / >>NF-kappa B signaling pathway; / >>Ubiquitin mediated proteolysis; / >>MicroRNAs in cancer
FunctionCatalytic activity:ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.,Function:Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Essential for nuclear architecture and chromosome segregation.,pathway:Protein modification; protein sumoylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with HIPK1, HIPK2 and PPM1J (By similarity). Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TCF3, TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with human adenovirus E1A and human herpesvirus 6 IE2. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B.,tissue specificity:Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.,
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