U-Blot™ Prestained Protein Ladder(10-180kDa)
Product details
U-Blot™ Prestained Protein Ladder (10-180k Da) contains 10 highly purified and prestained recombinant proteins (10, 17, 25, 34, 43, 55, 75, 95, 130, 180 kDa). The 75 kDa band is orange-red, and the 25 kDa band is green. It is suitable for SDS-PAGE polyacrylamide gel electrophoresis and immunoblotting.
Applications
The molecular weight range allows dynamic observation of protein electrophoresis and clear determination of the target protein’s molecular weight. After SDS-PAGE electrophoresis, the protein can be transferred to a PVDF (or NC) membrane, where a clear colored band indicates transfer efficiency in Western Blot experiments. The recommended sample volume is 5 to 10 μl.
Specifications
| Storage Buffer | |
| 20mM Tris-H3PO4 (pH7.5) | |
| 2mM EDTA | |
| 1.5% (W/V) SDS | |
| 15% (W/V) Glycerol | |
| 4M Urea | |
| 3mM DTT | |
| 0.1% (V/V) Proclin300 | |
| Shelf life: | Stable at -20°C for 36 months ; 4°C for 3 months; 25°C for 4 weeks |
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