Product details
U-Blot™ Cytoplasmic and Nuclear Extraction Kit for Cells is composed of optimized cytoplasmic extraction buffer, nuclear protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly separate native cytosol and nuclear proteins from mammalian cells and protoplasts of plant, bacteria, yeast and fungus. Due to the use of the protein extraction filter cartridges separation of cytoplasmic and nuclear proteins can be accomplished in less than 15 min.
Application
The kit is designed to rapidly extract native cytoplasmic and nuclear proteins from cultured cells for applications such as SDS-PAGE, immunoblotting, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels and other applications. This kit provides the most rapid method currently available for fractionation of native cytoplasmic and nuclear proteins.
