Product details
Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable. YD-016 provides a detergent-free, rapid and gentle way for extracting proteins from microbes with thick and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, gram positive and negative bacteria, insect eggs and microalgae. This kit contains optimized detergent-free and EDTA-free protein extraction buffers. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 2-4 mg/ml with un-biased protein representation. This kit features a single tube protocol and works well for both log phase and stationary phase microbes. The materials provided are sufficient for 50 extractions.
Application
Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA and enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification.
