Product details
Endoplasmic reticulum (ER) is a major membranous structure that functionally connects nuclear membrane and plasma membrane. Liver is an ER-rich organ and has been used for isolation of ER for many decades. Traditional method for isolating ER is based on density gradient ultracentrifugation. The protocol requires large amount of starting material and the methods are tedious and time consuming with significant cross- contamination. Currently, all commercial kits for ER isolation are based on the methods developed in 1970s of last century. Unlike any other ER isolation kit in the market, this kit employees a patented spin-column based technology that is simple, rapid and requires only small amount of tissue. This kit can differentially precipitate native ER (mainly rough ER) from cultured frozen liver tissues without using a Dounce homogenizer and ultracentrifugation. The whole protocol can be done in about 2h.
