U-Blot™ Protein Extraction Kit for Fixed and Embedded Tissues

Formalin-fixed and paraffin-embedded (FFPE) tissues are widely available and serve as a potential rich source for biomedical research. However, protein extraction from FFPE tissue is very challenging due to formalin mediated molecular cross-linking. Traditional methods for protein extraction from FFPE tissues involve repeated deparaffinization and rehydration using organic solvents followed by protein extraction at high temperature with or without optional sonication step. These methods, though relatively effective in some cases, are tedious and time consuming (3-4 h). FE-025 provides a simple and rapid way to extract protein from FFPE tissue without organic solvent deparaffinization. The whole procedure can be completed in less than one hour with a protein yield of 1-2 mg/ml.

Application

Extracted proteins can be used for many downstream applications such as SDS-PAGE, Western blotting, ELISA, immunoprecipitation and proteomic studies.