Product details
The intestines are long and continuous tubes that run from stomach to anus. Mesentery is a fold of tissue that attaches intestines to the body wall. Structurally intestines and mesentery are very different. However, from protein extraction point of view, they share one thing in common, e.g. they are very tough and hard to homogenize. It is also very difficult to lyse cells in the tissues for total protein extraction. The traditional solution-based protein extraction method such as RIPA is inefficient and protein yield is very low. The profile of extracted protein using traditional method is usually incomplete. This kit provides a highly efficient method for total protein extraction from human or animal intestines and mesentery tissues by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffer. The kit features a simple and rapid single tube protocol and optimized buffers for intestine and mesentery tissues. The researchers have the option to choose either denaturing cell lysis buffer or native cell lysis buffer for specific downstream applications. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.
Application
Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification. The salts and detergents in the extracted protein sample should be removed prior to mass spectrometry analysis.
