UHRF1 Monoclonal Antibody
Ucallm_WM0549-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],
Specifications
| Size | 50μL/100μL |
| Target | UHRF1 |
| Reactivity | Human |
| Applications | WB, IF |
| MW(Calculated) | 90kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IF 1:100-1:500; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 14B3 |
| Immunogen | Purified recombinant human UHRF1 (N-terminus) protein fragments expressed in E.coli. |
| Specificity | UHRF1 Monoclonal Antibody detects endogenous levels of UHRF1 protein. |
| Gene Name | UHRF1 |
| Protein Name | E3 ubiquitin-protein ligase UHRF1 |
| Other Name | UHRF1; / ICBP90; / NP95; / RNF106; / E3 ubiquitin-protein ligase UHRF1; / Inverted CCAAT box-binding protein of 90 kDa; / Nuclear protein 95; / Nuclear zinc finger protein Np95; / HuNp95; / hNp95; / RING finger protein 106; / Transcription factor ICBP90; / Ubiquit |
| Species | Human |
| Gene ID | 29128 |
| Uniprot | Q96T88, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8VDF2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions. |
| Tissue Expression | Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Expressed in fetal thymus, liver and kidney.,Domain:The RING finger is required for ubiquitin ligase activity.,Domain:The YDG domain mediates the interaction with histone H3.,Function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 1 YDG domain.,similarity:Contains 2 RING-type zinc fingers.,subunit:Interacts with histones H3, H1 and H2B (By similarity). Interacts with HDAC1, but not with HDAC2. Interacts with UHRF1BP1. Binds methylated CpG containing oligonucleotides.,tissue specificity:Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer., |
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