SKU: WP14960 Categories: ,

USP48 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP14960-100
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Product details

Background:

This gene encodes a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin. Enzymes in peptidase family C19 are involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetUSP48
ReactivityHuman, Mouse, Rat
ApplicationsIHC, IF, WB, ELISA
MW(Calculated)
MW(Observed)120kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human USP48. AA range:631-680
SpecificityUSP48 Polyclonal Antibody detects endogenous levels of USP48 protein.
Gene NameUSP48
Protein NameUbiquitin carboxyl-terminal hydrolase 48
Other NameUSP48; / USP31; / Ubiquitin carboxyl-terminal hydrolase 48; / Deubiquitinating enzyme 48; / Ubiquitin thioesterase 48; / Ubiquitin-specific-processing protease 48
SpeciesHuman
Gene ID84196
UniprotQ86UV5,
Species.1Mouse
Gene ID.1170707
Uniprot.1Q3V0C5,
Species.2Rat
Gene ID.2362636
Uniprot.2Q76LT8
Cellular LocalizationCytoplasm . Nucleus .
Tissue ExpressionWidely expressed.
Signaling_pathway
Research Areas
FunctionCatalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,Caution:Was named USP31 by some authors (PubMed:15354349 and PubMed:17081983).,Function:Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at post-synaptic sites.,sequence Caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the peptidase C19 family.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 3 DUSP domains.,subunit:Interacts with TRAF2 and RELA.,tissue specificity:Widely expressed.,
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