Name: V-ATPase H Polyclonal Antibody
SKU: WP14978
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],

Specifications

Target	V-ATPase H
Reactivity	Human, Mouse
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	55kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human ATP6V1H. AA range:341-390
Sequence
Specificity	V-ATPase H Polyclonal Antibody detects endogenous levels of V-ATPase H protein.
Gene Name	ATP6V1H
Protein Name	V-type proton ATPase subunit H
Other Name	ATP6V1H; / CGI-11; / V-type proton ATPase subunit H; / V-ATPase subunit H; / Nef-binding protein 1; / NBP1; / Protein VMA13 homolog; / V-ATPase 50/57 kDa subunits; / Vacuolar proton pump subunit H; / Vacuolar proton pump subunit SFD
Species	Human
Gene ID-1	51606
Uniprot	Q9UI12,
Species.1	Mouse
Gene ID-2	108664
Uniprot.1	Q8BVE3
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
Cellular Localization	Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein .
Tissue Expression	Widely expressed.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Transport and catabolism >> Lysosome;Organismal Systems >> Nervous system >> Synaptic vesicle cycle;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>Oxidative phosphorylation; / >>Metabolic pathways; / >>Lysosome; / >>Phagosome; / >>mTOR signaling pathway; / >>Synaptic vesicle cycle; / >>Vibrio cholerae infection; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Tuberculosis; / >>Human papillomavirus infection; / >>Rheumatoid arthritis
Function	Function:Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.,similarity:Belongs to the V-ATPase H subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein and AP2M1.,tissue specificity:Widely expressed.,
RRID

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V-ATPase H Polyclonal Antibody

Product details

Specifications

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V-ATPase H Polyclonal Antibody

Product details

Specifications

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