Name: U-Blot® Vimentin Rabbit mAb
SKU: WR4297
Price: 268 USD
Availability: InStock

U-Blot® Vimentin Rabbit mAb

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

WR4297 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.

Western blot analysis of lysates from Hela cells, using Vimentin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (RS0002,immunoway) was used to detect the antibody.

WR4297 Western blot analysis of lysates from Hela cells, using Vimentin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4297 Rat skin

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4297-50

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Product details

Background:This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],

Specifications

Size	50μL/100μL
Target	Vimentin
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	54kD
MW(Observed)	54kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:5000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0495R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	VIM
Protein Name	Vimentin
Other Name	VIM; / Vimentin
Species	Human
Gene ID	7431
Uniprot	P08670,
Species.1	Mouse
Gene ID.1	22352
Uniprot.1	P20152,
Species.2	Rat
Gene ID.2	81818
Uniprot.2	P31000
Cellular Localization	Cytoplasm
Tissue Expression	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Signaling_pathway	Human Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas	>>Epstein-Barr virus infection; / >>MicroRNAs in cancer
Function	Function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence Caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.,