WAVE1 Polyclonal Antibody
Ucallm_WP15009-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | WAVE1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human WAVE1. AA range:91-140 |
| Specificity | WAVE1 Polyclonal Antibody detects endogenous levels of WAVE1 protein. |
| Gene Name | WASF1 |
| Protein Name | Wiskott-Aldrich syndrome protein family member 1 |
| Other Name | WASF1; / KIAA0269; / SCAR1; / WAVE1; / Wiskott-Aldrich syndrome protein family member 1; / WASP family protein member 1; / Protein WAVE-1; / Verprolin homology domain-containing protein 1 |
| Species | Human |
| Gene ID | 8936 |
| Uniprot | Q92558, |
| Species.1 | Mouse |
| Gene ID.1 | 83767 |
| Uniprot.1 | Q8R5H6, |
| Species.2 | Rat |
| Gene ID.2 | 294568 |
| Uniprot.2 | Q5BJU7 |
| Cellular Localization | Cytoplasm, cytoskeleton . Cell junction, synapse . Cell junction, focal adhesion . Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity). . |
| Tissue Expression | Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis |
| Research Areas | >>Adherens junction; / >>Fc gamma R-mediated phagocytosis; / >>Regulation of actin cytoskeleton; / >>Bacterial invasion of epithelial cells; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Choline metabolism in cancer |
| Function | Domain:Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.,Function:Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton.,similarity:Belongs to the SCAR/WAVE family.,similarity:Contains 1 WH2 domain.,subcellular location:Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.,subunit:Component of the WAVE1 complex composed of ABI2, CYFIP2, C3orf10/HSPC300, NCKAP1 and WASF1/WAVE1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1 (By similarity). Binds actin and the Arp2/3 complex. Interacts with BAIAP2.,tissue specificity:Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood., |
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