Name: Wnt-5a Monoclonal Antibody
SKU: WM0466
Price: 268 USD
Availability: InStock

Product details

Background:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],

Specifications

Target	Wnt-5a
Reactivity	Human
Applications	WB, IHC, IF, ELISA
MW(Calculated)	42kD
MW(Observed)
Host Species	Mouse
Isotype
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	Affinity purification
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	14H7
Immunogen	Purified recombinant fragment of Wnt-5a expressed in E. Coli.
Sequence
Specificity	Wnt-5a Monoclonal Antibody detects endogenous levels of Wnt-5a protein.
Gene Name	WNT5A
Protein Name	Protein Wnt-5a
Other Name	WNT5A; / Protein Wnt-5a
Species	Human
Gene ID-1	7474
Uniprot	P41221,
Species.1	Mouse
Gene ID-2
Uniprot.1	P22725
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012],
Cellular Localization	Secreted, extracellular space, extracellular matrix . Secreted .
Tissue Expression	Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level) (PubMed:15735754). Detected in neonate heart and lung (PubMed:8288227).
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Basal cell carcinoma;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>mTOR signaling pathway; / >>Wnt signaling pathway; / >>Axon guidance; / >>Hippo signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells; / >>Melanogenesis; / >>Cushing syndrome; / >>Alzheimer disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Human papillomavirus infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Basal cell carcinoma; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer
Function	Function:Ligand for members of the frizzled family of seven transmembrane receptors.,Function:Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.,PTM:Glycosylation is necessary for secretion but not for activity.,PTM:Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding.,similarity:Belongs to the Wnt family.,subunit:Interacts with PORCN.,tissue specificity:Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells (at protein level).,
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Wnt-5a Monoclonal Antibody

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Wnt-5a Monoclonal Antibody

Product details

Specifications

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