Price range: $268.00 through $328.00
Background:
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
| Size | 50μL/100μL |
| Target | GAPDH |
| Reactivity | Human, Mouse, Rat, Yeast |
| Applications | IHC, WB |
| MW(Calculated) | |
| MW(Observed) | 37kD |
| Host Species | Mouse |
| Isotype | IgG1,kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC1:50-200; WB 1:1000-2000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | 10F8 |
| Immunogen | Synthesized peptide derived from Yeast GAPDH |
| Specificity | This antibody detects endogenous levels of Yeast GAPDH |
| Gene Name | Yeast GAPDH |
| Protein Name | Yeast GAPDH |
| Other Name | |
| Species | Human |
| Gene ID | |
| Uniprot | P00360 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function |
