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Background:This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014],
| Target | ZO1 |
| Reactivity | Human, Mouse, Rat, Pig |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 195kD |
| MW(Observed) | 220kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Source | |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | PT0639R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Sequence | |
| Specificity | Endogenous |
| Gene Name | TJP1 ZO1 |
| Protein Name | Tight junction protein ZO-1 (Tight junction protein 1) (Zona occludens protein 1) (Zonula occludens protein 1) |
| Other Name | |
| Species | Human |
| Gene ID-1 | 7082 |
| Uniprot | Q07157, |
| Species.1 | Mouse |
| Gene ID-2 | |
| Uniprot.1 | P39447 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014], |
| Cellular Localization | Membrane |
| Tissue Expression | The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cellular community - eukaryotes >> Gap junction |
| Research Areas | >>Adherens junction; / >>Tight junction; / >>Gap junction; / >>Vibrio cholerae infection; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Pathogenic Escherichia coli infection |
| Function | Domain:The second PDZ domain mediates interaction with GJA12.,Function:The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.,PTM:Phosphorylated.,similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 1 ZU5 domain.,similarity:Contains 3 PDZ (DHR) domains.,subcellular location:Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.,subunit:Interacts with HSPA4 and KIRREL1 (By similarity). Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with occludin, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1.,tissue specificity:The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules., |
| RRID |
