Collagen III Rabbit Monoclonal antibody

Collagen III Rabbit Monoclonal antibody

Background:collagen type III alpha 1 chain(COL3A1) Homo sapiens This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.

Item Details
Collagen III Rabbit mAb WR4091
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:5000 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 138 kDa
Observed band size 150 kDa

Boost your connective tissue and collagen research with the Collagen III Rabbit Monoclonal antibody (WR4091)—a Google-optimized, rigorously validated tool for specific COL3A1 detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, HUVEC, etc.), this antibody delivers clear banding (138 kDa predicted / 150 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:5000 dilution) and a robust Dylight 800-conjugated detection system.

Ideal for studying type III collagen in extensible tissues (skin, lung, vascular system) or researching Ehlers-Danlos syndrome/aneurysm-related mutations, this trusted Collagen III Rabbit Monoclonal antibody enhances assay reproducibility. Order WR4091 today to advance your collagen biology experiments with a SEO-verified, high-performance research tool tailored for connective tissue studies.

Leave a Comment

Your email address will not be published. Required fields are marked *