Price range: $268.00 through $328.00
Background:This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | HES1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | 30kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:4000-1:20000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0857R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | HES1 BHLHB39 HL HRY |
| Protein Name | Transcription factor HES-1 (Class B basic helix-loop-helix protein 39) (bHLHb39) (Hairy and enhancer of split 1) (Hairy homolog) (Hairy-like protein) (hHL) |
| Other Name | |
| Species | Human |
| Gene ID | 3280 |
| Uniprot | Q14469, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P35428, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q04666 |
| Cellular Localization | Nucleus. |
| Tissue Expression | Kidney,Prostate,Skin, |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Environmental Information Processing >> Signal transduction >> Notch signaling pathway |
| Research Areas | >>Fanconi anemia pathway; / >>Notch signaling pathway; / >>Maturity onset diabetes of the young; / >>Human papillomavirus infection; / >>Epstein-Barr virus infection; / >>Pathways in cancer; / >>Breast cancer |
| Function | Domain:Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).,Domain:The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.,Domain:The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.,Function:Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 Orange domain.,subunit:Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1, and more weakly with TLE2. Interacts with HES6 (By similarity). Interacts with SIRT1., |
