SKU: WM0313 Categories: ,

MEK Kinase-2 Monoclonal Antibody

Price range: $268.00 through $328.00

SKU: WM0313-50
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Product details

Background:
The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
TargetMAP3K2
ReactivityHuman
ApplicationsWB, IF, ELISA
MW(Calculated)70kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number6C10
ImmunogenPurified recombinant fragment of human MEK Kinase-2 expressed in E. Coli.
SpecificityMEK Kinase-2 Monoclonal Antibody detects endogenous levels of MEK Kinase-2 protein.
Gene NameMAP3K2 MAPKKK2 MEKK2
Protein NameMitogen-activated protein kinase kinase kinase 2
Other NameMEK kinase 2; / MAP3K2; / MAPKKK2; / MEKK2; / Mitogen-activated protein kinase kinase kinase 2; / MAPK/ERK kinase kinase 2; / MEKK 2
SpeciesHuman
Gene ID10746
UniprotQ9Y2U5,
Species.1Mouse
Gene ID.1
Uniprot.1Q61083
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Upon EGF stimulation, translocates into the nucleus.
Tissue ExpressionBrain,Platelet,T-cell,Teratocarcinoma,Whole embryo,
Signaling_pathwayCellular Processes >> Cellular community - eukaryotes >> Gap junction;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway
Research Areas>>MAPK signaling pathway; / >>Gap junction; / >>GnRH signaling pathway
FunctionCatalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-524.,Function:Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 OPR domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Upon EGF stimulation, translocates into the nucleus.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes.,
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