Price range: $268.00 through $328.00
Background:
The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | DHODH |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 48kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DHODH. AA range:211-260 |
| Specificity | DHODH Polyclonal Antibody detects endogenous levels of DHODH protein. |
| Gene Name | DHODH |
| Protein Name | Dihydroorotate dehydrogenase |
| Other Name | DHODH; / Dihydroorotate dehydrogenase; / quinone; / mitochondrial; / DHOdehase; / Dihydroorotate oxidase |
| Species | Human |
| Gene ID | 1723 |
| Uniprot | Q02127, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | O35435 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein . |
| Tissue Expression | Skin,Testis, |
| Signaling_pathway | |
| Research Areas | >>Pyrimidine metabolism; / >>Metabolic pathways; / >>Biosynthesis of cofactors |
| Function | Catalytic activity:(S)-dihydroorotate + O(2) = orotate + H(2)O(2).,cofactor:Binds 1 FAD per subunit.,pathway:Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.,similarity:Belongs to the dihydroorotate dehydrogenase family., |
