Price range: $268.00 through $328.00
Background:
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | DGK-ε |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKE. AA range:161-210 |
| Specificity | DGK-ε Polyclonal Antibody detects endogenous levels of DGK-ε protein. |
| Gene Name | DGKE |
| Protein Name | Diacylglycerol kinase epsilon |
| Other Name | DGKE; / DAGK5; / Diacylglycerol kinase epsilon; / DAG kinase epsilon; / Diglyceride kinase epsilon; / DGK-epsilon |
| Species | Human |
| Gene ID | 8526 |
| Uniprot | P52429, |
| Species.1 | Mouse |
| Gene ID.1 | 56077 |
| Uniprot.1 | Q9R1C6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Membrane; Single-pass membrane protein . Cytoplasm . |
| Tissue Expression | Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level). |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway |
| Research Areas | >>Glycerolipid metabolism; / >>Glycerophospholipid metabolism; / >>Metabolic pathways; / >>Phosphatidylinositol signaling system; / >>Phospholipase D signaling pathway; / >>Choline metabolism in cancer |
| Function | Catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,Function:Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Expressed predominantly in testis., |
