Product details

Background:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

Specifications

Target	ERK 1/2
Reactivity	Human, Mouse, Rat
Applications	IF, WB, IHC, ELISA, CHIP
MW(Calculated)
MW(Observed)	42,44kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IF 1:50-200; WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; Not yet tested in other applications; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human p44/42 MAPK. AA range:330-379
Sequence
Specificity	ERK 1/2 Polyclonal Antibody detects endogenous levels of ERK 1/2 protein.
Gene Name	MAPK1/MAPK3
Protein Name	Mitogen-activated protein kinase 3
Other Name	MAPK1; / ERK2; / PRKM1; / PRKM2; / Mitogen-activated protein kinase 1; / MAP kinase 1; / MAPK 1; / ERT1; / Extracellular signal-regulated kinase 2; / ERK-2; / MAP kinase isoform p42; / p42-MAPK; / Mitogen-activated protein kinase 2; / MAP kinase 2; / MAPK 2; / MAPK3; / MAPK3; / ERK1; / PRKM3; / Mitogen-activated protein kinase 3; / MAP kinase 3; / MAPK 3; / ERT2; / Extracellular signal-regulated kinase 1; / ERK-1; / Insulin-stimulated MAP2 kinase; / MAP kinase isoform p44; / p44-MAPK; / Microtubule-associated protein 2 kinase; / p
Species	Human
Gene ID-1	5594;5595
Uniprot	P27361;P28482,
Species.1	Mouse
Gene ID-2	26417;26413,
Uniprot.1
Species.2	Rat
Gene ID-3	50689;116590
Uniprot.2	P21708;P63086
Organism-4
Gene ID-4
SwissProt-4
Background	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],
Cellular Localization	Cytoplasm . Nucleus. Membrane, caveola . Cell junction, focal adhesion . Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity). .
Tissue Expression	Epithelium,Eye,Hepatoma,Human cervix,Lymph,
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Gap junction;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> GnRH signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Thyroid cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Bladder cancer;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>Platinum drug resistance; / >>MAPK signaling pathway; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Oocyte meiosis; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apoptosis; / >>Cellular senescence; / >>Adrenergic signaling in cardiomyocytes; / >>Vascular smooth muscle contraction; / >>TGF-beta signaling pathway; / >>Axon guidance; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Osteoclast differentiation; / >>Focal adhesion; / >>Adherens junction; / >>Gap junction; / >>Signaling pathways regulating pluripotency of stem cells; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>IL-17 signaling pathway; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>TNF signaling pathway; / >>Circadian entrainment; / >>Long-term potentiation; / >>Neurotrophin signaling pathway; / >>Retrograde endocannabinoid signaling; / >>Glutamatergic synapse; / >>Cholinergic synapse; / >>Serotonergic synapse; / >>Long-term depression; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>GnRH signaling pathway; / >>Progesterone-mediated oocyte maturation; / >>Estrogen signaling pathway; / >>Melanogenesis; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Oxytocin signaling pathway; / >>Relaxin signaling pathway; / >>Parathyroid hormone synthesis, secretion and action; / >>GnRH secretion; / >>Type II diabetes mellitus; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Cushing syndrome; / >>Growth hormone synthesis, secretion and action; / >>Aldosterone-regulated sodium reabsorption; / >>Alzheimer disease; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Alcoholism; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Pertussis; / >>Yersinia infection; / >>Leishmaniasis; / >>Chagas disease; / >>Toxoplasmosis; / >>Tuberculosis; / >>Hepatitis C; / >>Hepatitis B; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Thyroid cancer; / >>Melanoma; / >>Bladder cancer; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Lipid and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF.,Function:Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).,PTM:Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.,
RRID	AB_3096063

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ERK 1/2 Polyclonal Antibody

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ERK 1/2 Polyclonal Antibody

Product details

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