Product details

Background:

serine/threonine kinase 3(STK3) Homo sapiens This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],

Specifications

Size	50μL/100μL
Target	Krs-1/2
Reactivity	Human, Mouse
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	56kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human Mst1/2. AA range:149-198
Specificity	Krs-1/2 Polyclonal Antibody detects endogenous levels of Krs-1/2 protein.
Gene Name	STK3/STK4
Protein Name	Serine/threonine-protein kinase 3/4
Other Name	STK3; / KRS1; / MST2; / Serine/threonine-protein kinase 3; / Mammalian STE20-like protein kinase 2; / MST-2; / STE20-like kinase MST2; / Serine/threonine-protein kinase Krs-1; / STK4; / KRS2; / MST1; / Serine/threonine-protein kinase 4; / Mammalian STE20-like prot
Species	Human
Gene ID	6788;6789
Uniprot	Q13188;Q13043,
Species.1	Mouse
Gene ID.1	56274;58231
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm . Nucleus . The caspase-cleaved form cycles between nucleus and cytoplasm (PubMed:19525978, PubMed:11278283). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (PubMed:19525978). .
Tissue Expression	Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
Signaling_pathway	Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway - multiple species;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Hippo signaling pathway; / >>Hippo signaling pathway - multiple species
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1.,Function:Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Phosphorylates NKX2-1 (By similarity). Phosphorylates and activates LATS1 and LATS2.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SARAH domain.,subcellular location:The caspase-cleaved form cycles between nucleus and cytoplasm.,subunit:Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1, which leads to enzyme activation.,tissue specificity:Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.,

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Krs-1/2 Polyclonal Antibody

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Krs-1/2 Polyclonal Antibody

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