Price range: $268.00 through $328.00
Background:
Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Macroglobulin α-2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 170kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human A2M. AA range:871-920 |
| Specificity | Macroglobulin α-2 Polyclonal Antibody detects endogenous levels of Macroglobulin α-2 protein. |
| Gene Name | A2M |
| Protein Name | Alpha-2-macroglobulin |
| Other Name | A2M; / CPAMD5; / FWP007; / Alpha-2-macroglobulin; / Alpha-2-M; / C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 |
| Species | Human |
| Gene ID | 2 |
| Uniprot | P01023 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Secreted . |
| Tissue Expression | Secreted in plasma. |
| Signaling_pathway | Organismal Systems >> Immune system >> Complement and coagulation cascades |
| Research Areas | >>Complement and coagulation cascades |
| Function | developmental stage:Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.,Function:Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.,online information:Alpha-2 macroglobulin entry,similarity:Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.,subunit:Homotetramer; disulfide-linked.,tissue specificity:Plasma., |
