AMPK α1 Rabbit Monoclonal Antibody

AMPK α1 Rabbit Monoclonal Antibody

Background:The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Item Details
AMPK a1 Rabbit mAb WR4079
0.22 Nitrocellulose Membrane W3N21
pre-staining standard protein Marker(10-180kDa) W2100
Lane Cell Line
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Parameter Details
SDS-PAGE Separation Range 4-20%
Primary Antibody Incubation Condition 4°C, over night
Primary Antibody Dilution 1:2500
Secondary Antibody Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446)
Type Size
Predicted band size 64 kDa
Observed band size 64 kDa

If you’re researching cellular energy sensing, metabolic enzyme regulation, or cell protection against ATP-depleting stress—you need an AMPK α1 antibody that’s consistent across your experiments.The AMPK α1 Rabbit Monoclonal Antibody (WR4079) delivers exactly that: it’s validated across 14 human/mouse/rat cell lines (HL60, HUVEC, HCT116, and more), so you never waste time troubleshooting cell-specific signal drops. Its 1:2500 dilution ratio cuts reagent costs without weakening detection.

Most critically, it hits the precise 64 kDa band (predicted = observed)—ensuring you’re detecting the functional AMPK α1 subunit that drives energy homeostasis and metabolic enzyme regulation (the core of your research focus).Search “AMPK α1 Rabbit Monoclonal Antibody WR4079” or “AMPK α1 antibody for cellular energy sensor & metabolic regulation” to access the product page: grab optimized protocols, request a trial sample, and advance your AMPK α1 studies without antibody-related delays.

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