Antibody Basics Corner
BY December 3, 2025

Cyclin E1 Rabbit Monoclonal Antibody

Cyclin E1 Rabbit Monoclonal Antibody

Background：The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.

Item Details
Cyclin E1 Rabbit mAb WR4078
0.22 Nitrocellulose Membrane W3N21
pre-staining standard protein Marker(10-180kDa) W2100
Lane Cell Line
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Parameter Details
SDS-PAGE Separation Range 4-20%
Primary Antibody Incubation Condition 4°C, over night
Primary Antibody Dilution 1:2500
Secondary Antibody Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446)
Type Size
Predicted band size 47 kDa
Observed band size 47 kDa

If you’re studying cell cycle G1/S transition or tumorigenesis linked to Cyclin E1 overexpression, you need an antibody that delivers precise, consistent results. The Cyclin E1 Rabbit Monoclonal Antibody (WR4078) is validated across 14 human/mouse/rat cell lines (HL60, HUVEC, HCT116, etc.)—no more hit-or-miss signal across your experimental panels—with a cost-saving 1:2500 dilution that doesn’t sacrifice detection strength.

Critically, it hits the exact 47 kDa band (predicted = observed), so you’re detecting the functional Cyclin E1-CDK2 complex that drives cell cycle progression and tumor chromosome instability. Search “Cyclin E1 Rabbit Monoclonal Antibody WR4078” or “Cyclin E1 antibody for cell cycle G1/S transition & tumorigenesis” to access protocols, request samples, and advance your Cyclin E1 research without antibody troubleshooting

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