GLSK Rabbit Monoclonal Antibody

GLSK Rabbit Monoclonal Antibody

Background:This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.

 

Item Details
Glutaminase C Rabbit mAb WR4082
0.22 Nitrocellulose Membrane W3N21
Item Details
pre-staining standard protein Marker (10-180kDa):W2100
Lane Number Cell Information
Lane1 HL60 – Human promyelocytic leukemia cell
Lane2 HUVEC – Human umbilical vein endothelial cell
Lane3 Human CAF – Human cancer-associated fibroblast
Lane4 HCT116 – Human colorectal carcinoma
Lane5 A549 – Human lung carcinoma
Lane6 Jurkat – Human T lymphocyte leukemia
Lane7 HT22 – Mouse hippocampal neuronal
Lane8 Thp-1 – Human monocytic leukemia
Lane9 HK-2 – Human proximal tubular epithelial
Lane10 PC-12 – Rat adrenal pheochromocytoma
Lane11 3T3-L1 – Mouse embryonic fibroblast cells
Lane12 PC-3 – Human prostate adenocarcinoma
Lane13 U-14 – Mouse cervical carcinoma
Lane14 293F – HEK293 derivative, adapted for suspension culture
Item Details
SDS-PAGE separation Various whole cell lysates were separated by 4-20% SDS-PAGE
Primary antibody conditions Used at 4°C, over night with a 1:2500 dilution
Detection antibody Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446)
Item Band Size
Predicted band size 68 kDa
Observed band size 55-65 kDa

Boost your mitochondrial glutaminase research with the Glutaminase C Rabbit Monoclonal Antibody (WR4082)—a Google-optimized, rigorously validated tool for specific target detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, HT22, etc.), this antibody delivers reliable banding (68 kDa predicted / 55-65 kDa observed) via 4-20% SDS-PAGE, with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated detection system.

Ideal for studies of energy metabolism, glutamate neurotransmitter synthesis, or renal acid-base balance—key functions of the K-type mitochondrial glutaminase—this trusted antibody enhances assay reproducibility. Order the Glutaminase C Rabbit Monoclonal Antibody (WR4082) today to advance your glutaminase research with a SEO-friendly, peer-backed tool.

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