Background：The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
| Item | Details |
|---|---|
| PCNA Rabbit mAb[PT0224R] | WR4120 |
| 0.22 Nitrocellulose Membrane | W3N21 |
| Item | Details |
|---|---|
| pre-staining standard protein Marker | (10-180kDa):W2100 |
| Lane Number | Cell Information |
|---|---|
| Lane1 | HL60 – Human promyelocytic leukemia cell |
| Lane2 | HUVEC – Human umbilical vein endothelial cell |
| Lane3 | Human CAF – Human cancer-associated fibroblast |
| Lane4 | HCT116 – Human colorectal carcinoma |
| Lane5 | A549 – Human lung carcinoma |
| Lane6 | Jurkat – Human T lymphocyte leukemia |
| Lane7 | HT22 – Mouse hippocampal neuronal |
| Lane8 | Thp-1 – Human monocytic leukemia |
| Lane9 | HK-2 – Human proximal tubular epithelial |
| Lane10 | PC-12 – Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 – Mouse embryonic fibroblast cells |
| Lane12 | PC-3 – Human prostate adenocarcinoma |
| Lane13 | U-14 – Mouse cervical carcinoma |
| Lane14 | 293F – HEK293 derivative, adapted for suspension culture |
| Item | Details |
|---|---|
| SDS-PAGE separation | Various whole cell lysates were separated by 4-20% SDS-PAGE |
| Primary antibody conditions | Used at 4°C, over night with a 1:2500 dilution |
| Detection antibody | Dylight 800-conjugated Goat anti-Rabbit antibody (Cat:WS0446) |
| Item | Band Size |
|---|---|
| Predicted band size | 29 kDa |
| Observed band size | 36 kDa |
Elevate your DNA replication and DNA damage repair research with the PCNA Rabbit Monoclonal Antibody (WR4120, PCNA Rabbit mAb)—a Google-optimized, rigorously validated tool for specific PCNA detection. Tested across 14+ human, mouse, and rat cell lines (HL60, HCT116, A549, etc.), this antibody delivers clear, consistent banding (29 kDa predicted / 36 kDa observed) via 4-20% SDS-PAGE, paired with optimized conditions (4°C overnight incubation, 1:2500 dilution) and a robust Dylight 800-conjugated Goat anti-Rabbit detection system.
Ideal for studying PCNA’s roles as a DNA polymerase delta cofactor, a homotrimer that enhances leading strand synthesis processivity during DNA replication, or its ubiquitination-mediated function in the RAD6-dependent DNA repair pathway after DNA damage, this trusted PCNA Rabbit Monoclonal Antibody boosts assay reproducibility for DNA biology, genome stability, and cancer research. Order WR4120 today to advance your PCNA-focused experiments with a SEO-verified, high-performance research tool tailored for DNA replication and damage repair studies.
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