Price range: $268.00 through $328.00
Background:
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | AATC |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, WB |
| MW(Calculated) | |
| MW(Observed) | 42kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; WB 1:500-2000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human AATC |
| Specificity | This antibody detects endogenous levels of human AATC |
| Gene Name | GOT1 |
| Protein Name | AATC |
| Other Name | Aspartate aminotransferase, cytoplasmic; / Glutamate oxaloacetate transaminase 1; / Transaminase A |
| Species | Human |
| Gene ID | 2805 |
| Uniprot | P17174 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Brain,Liver,Lung, |
| Signaling_pathway | |
| Research Areas | >>Arginine biosynthesis; / >>Alanine, aspartate and glutamate metabolism; / >>Cysteine and methionine metabolism; / >>Arginine and proline metabolism; / >>Tyrosine metabolism; / >>Phenylalanine metabolism; / >>Phenylalanine, tyrosine and tryptophan biosynthesis; / >>Metabolic pathways; / >>Carbon metabolism; / >>2-Oxocarboxylic acid metabolism; / >>Biosynthesis of amino acids |
| Function | Catalytic activity:L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.,cofactor:Pyridoxal phosphate.,miscellaneous:In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.,similarity:Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.,subunit:Homodimer., |
