Actin-α/γ Polyclonal Antibody
Ucallm_WP10939-50
Price range: $268.00 through $328.00
Product details
Background:
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Actin-α/γ |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 45kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Actin. AA range:21-70 |
| Specificity | Actin-α/γ Polyclonal Antibody detects endogenous levels of Actin-α/γ protein. |
| Gene Name | ACTC1; ACTA1; ACTG1; ACTG2 |
| Protein Name | Actin alpha cardiac muscle 1 |
| Other Name | ACTC1; / ACTC; / Actin; / alpha cardiac muscle 1; / Alpha-cardiac actin; / ACTG1; / ACTB; / ACTG; / Actin, cytoplasmic 2; / Gamma-actin; / ACTG2; / ACTA3; / ACTL3; / ACTSG; / Actin, gamma-enteric smooth muscle; / Alpha-actin-3; / Gamma-2-actin; / Smooth muscle gamma-actin |
| Species | Human |
| Gene ID | 58;70;71;72 |
| Uniprot | P68032;P63261;P63267;P68133, |
| Species.1 | Mouse |
| Gene ID.1 | 11464;11465;11468;11459, |
| Uniprot.1 | |
| Species.2 | Rat |
| Gene ID.2 | 29275;100361457;25365;29437 |
| Uniprot.2 | P68035;P63259;P63269;P68136 |
| Cellular Localization | Cytoplasm, cytoskeleton. |
| Tissue Expression | Muscle,Tongue, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Genetic Information Processing >> Chromosome >> ATP-dependent chromatin remodeling |
| Research Areas | >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Hypertrophic cardiomyopathy; / >>Dilated cardiomyopathy |
| Function | Disease:Defects in ACTC1 are the cause of cardiomyopathy dilated type 1R (CMD1R) [MIM:102540]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,Disease:Defects in ACTC1 are the cause of cardiomyopathy familial hypertrophic type 11 (CMH11) [MIM:612098]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.,Function:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.,miscellaneous:In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.,similarity:Belongs to the actin family.,subunit:Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others., |
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