U-Blot® ACTR1B Rabbit mAb
WR4644 Western Blot analysis of HepG2 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ACTR1B rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex. [provided by RefSeq, Aug 2008],
Specifications
| Size | 50μL/100μL |
| Target | ACTR1B |
| Reactivity | Human |
| Applications | WB, IHC, IF, ICC, FC, IP |
| MW(Calculated) | |
| MW(Observed) | 41kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-5000; IHC 1:100-300; ICC/IF 1:100-300; FC 1:100-300; IP 1:50-100 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0778R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ACTR1B; CTRN2 |
| Protein Name | Beta-centractin; Actin-related protein 1B; ARP1B |
| Other Name | ACTR1B; / CTRN2; / Beta-centractin; / Actin-related protein 1B; / ARP1B |
| Species | Human |
| Gene ID | 10120 |
| Uniprot | P42025, |
| Species.1 | Mouse |
| Gene ID.1 | 226977 |
| Uniprot.1 | Q8R5C5 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Tissue Expression | |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases |
| Research Areas | >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Salmonella infection |
| Function | Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome. |
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