Price range: $268.00 through $328.00
Background:
ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008],
| Target | ADP-GK |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 47kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADPGK. AA range:241-290 |
| Sequence | |
| Specificity | ADP-GK Polyclonal Antibody detects endogenous levels of ADP-GK protein. |
| Gene Name | ADPGK |
| Protein Name | ADP-dependent glucokinase |
| Other Name | ADPGK; / PSEC0260; / ADP-dependent glucokinase; / ADP-GK; / ADPGK; / RbBP-35 |
| Species | Human |
| Gene ID-1 | 83440 |
| Uniprot | Q9BRR6, |
| Species.1 | Mouse |
| Gene ID-2 | 72141 |
| Uniprot.1 | Q8VDL4 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008], |
| Cellular Localization | Secreted . |
| Tissue Expression | Brain,Heart,Kidney,Lymph node,Teratocarcinoma,Testis, |
| Signaling_pathway | |
| Research Areas | >>Glycolysis / Gluconeogenesis; / >>Metabolic pathways; / >>Carbon metabolism |
| Function | Catalytic activity:ADP + D-glucose = AMP + D-glucose 6-phosphate.,cofactor:Binds 1 magnesium ion per subunit.,Function:Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.,pathway:Carbohydrate degradation; glycolysis.,similarity:Belongs to the ADP-dependent glucokinase family.,similarity:Contains 1 ADPK (ADP-dependent kinase) domain.,subunit:Monomer., |
| RRID |
