ALY Polyclonal Antibody
Ucallm_WP11025-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | ALY |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 27kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human THOC4. AA range:121-170 |
| Specificity | ALY Polyclonal Antibody detects endogenous levels of ALY protein. |
| Gene Name | ALYREF |
| Protein Name | THO complex subunit 4 |
| Other Name | ALYREF; / ALY; / BEF; / THOC4; / THO complex subunit 4; / Tho4; / Ally of AML-1 and LEF-1; / Aly/REF export factor; / Transcriptional coactivator Aly/REF; / bZIP-enhancing factor BEF |
| Species | Human |
| Gene ID | 10189 |
| Uniprot | Q86V81, |
| Species.1 | Mouse |
| Gene ID.1 | 21681 |
| Uniprot.1 | O08583 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Nucleus speckle . Cytoplasm . Colocalizes with the core EJC, ALYREF/THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA (PubMed:19324961). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332). . |
| Tissue Expression | Expressed in a wide variety of cancer types. |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Genetic Information Processing >> Translation >> Nucleocytoplasmic transport |
| Research Areas | >>Nucleocytoplasmic transport; / >>mRNA surveillance pathway; / >>Spliceosome; / >>Amyotrophic lateral sclerosis; / >>Herpes simplex virus 1 infection |
| Function | Disease:Antibodies against THOC4 are found in sera of patients with systemic lupus erythematosus (SLE).,Function:Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Integral part of the THO/TREX complex that is recruited to transcribed genes and travels with the RNA polymerase during elongation. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.,PTM:Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.,subunit:Homomultimer. Binds LEF1 and RUNX1 (By similarity). Is part of several complexes involved in mRNA processing and export. Part of the heteromultimeric THO/TREX complex containing THOC1, THOC2, THOC3, THOC4 and UAP56. Associates with the spliceosome. Binds NXF1 and RBM8A directly and is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RBM8A, SRRM1, NXF1, RENT2, RENT3B and THOC4 (By similarity). Found in a mRNA splicing-dependent exon junction complex (EJC) with DEK, RBM8A, RNPS1, SRRM1 and THOC4. Binds UL54 from Herpes simplex type 1 (strain KOS). Found in a mRNP complex with RENT3A and RENT3B. Interacts with BAT1, RBM8A, RNPS1 and SRRM1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8., |
Please login to post questions
Related products
