Price range: $268.00 through $328.00
Background:
enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+)., function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity., similarity:Belongs to the alkaline ceramidase family., tissue specificity:Ubiquitously expressed. Highest expression in placenta.,
| Size | 50μL/100μL |
| Target | PHCA |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 35kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PHCA. AA range:171-220 |
| Specificity | APHC Polyclonal Antibody detects endogenous levels of APHC protein. |
| Gene Name | ACER3 |
| Protein Name | Alkaline ceramidase 3 |
| Other Name | ACER3; / APHC; / PHCA; / Alkaline ceramidase 3; / AlkCDase 3; / Alkaline CDase 3; / Alkaline dihydroceramidase SB89; / Alkaline phytoceramidase; / aPHC |
| Species | Human |
| Gene ID | 55331 |
| Uniprot | Q9NUN7, |
| Species.1 | Mouse |
| Gene ID.1 | 66190 |
| Uniprot.1 | Q9D099 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein . Golgi apparatus membrane; Multi-pass membrane protein . |
| Tissue Expression | Ubiquitously expressed. Highly expressed in placenta (PubMed:11356846). Expressed in erythrocytes (PubMed:20207939). |
| Signaling_pathway | |
| Research Areas | >>Sphingolipid metabolism; / >>Metabolic pathways |
| Function | enzyme regulation:Activated by Ca(2+) and inhibited by Zn(2+).,Function:Hydrolyzes only phytoceramide into phytosphingosine and free fatty acid. Does not have reverse activity.,similarity:Belongs to the alkaline ceramidase family.,tissue specificity:Ubiquitously expressed. Highest expression in placenta., |
