SKU: WP11097 Categories: ,

AQP4 Polyclonal Antibody

Price range: $268.00 through $328.00

SKU: WP11097-100
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Product details

Background:

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015],

Specifications

Size50μL/100μL
TargetAQP4
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)35kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human AQP4. AA range:204-253
SpecificityAQP4 Polyclonal Antibody detects endogenous levels of AQP4 protein.
Gene NameAQP4
Protein NameAquaporin-4
Other NameAQP4; / Aquaporin-4; / AQP-4; / Mercurial-insensitive water channel; / MIWC; / WCH4
SpeciesHuman
Gene ID361
UniprotP55087,
Species.1Mouse
Gene ID.111829
Uniprot.1P55088,
Species.2Rat
Gene ID.225293
Uniprot.2P47863
Cellular LocalizationCell membrane; Multi-pass membrane protein . Basolateral cell membrane; Multi-pass membrane protein . Endosome membrane . Cell membrane, sarcolemma; Multi-pass membrane protein . Cell projection . Activation of the vasopressin receptor AVPR1A triggers AQP4 phosphorylation at Ser-180 and promotes its internalization from the cell membrane. Detected on brain astrocyte processes and astrocyte endfeet close to capillaries. .
Tissue ExpressionDetected in skeletal muscle (PubMed:29055082). Detected in stomach, along the glandular base region of the fundic gland (at protein level) (PubMed:8601457). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary (PubMed:7559426, PubMed:8601457).
Signaling_pathway
Research Areas>>Vasopressin-regulated water reabsorption; / >>Bile secretion
FunctionDomain:Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).,Function:Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.,similarity:Belongs to the MIP/aquaporin (TC 1.A.8) family.,tissue specificity:Brain - muscle >> heart, kidney, lung, and trachea.,
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