Product details
Background:
ASAH1 rabbit pAb
Ucallm_WP6245-50
Price range: $268.00 through $328.00
Specifications
| Size | 50μL/100μL |
| Target | ASAH1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| MW(Calculated) | 43kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human ASAH1 |
| Specificity | This antibody detects endogenous levels of ASAH1 at Human, Mouse,Rat |
| Gene Name | ASAH1 ASAH HSD-33 HSD33 |
| Protein Name | Acid ceramidase (AC) (ACDase) (Acid CDase) (Acylsphingosine deacylase) (N-acylsphingosine amidohydrolase) (Putative 32 kDa heart protein) (PHP32) [Cleaved into: Acid ceramidase subunit alpha; Acid ceramidase subunit beta] |
| Other Name | |
| Species | Human |
| Gene ID | 427 |
| Uniprot | Q13510, |
| Species.1 | Mouse |
| Gene ID.1 | 11886 |
| Uniprot.1 | Q9WV54, |
| Species.2 | Rat |
| Gene ID.2 | 84431 |
| Uniprot.2 | Q6P7S1 |
| Cellular Localization | Lysosome . Secreted . Secretion is extremely low and localization to lysosomes is mannose-6-phosphate receptor-dependent. .; [Isoform 2]: Nucleus . Cytoplasm . A localization to the nucleus and the cytoplasm has also been reported for ASAH1, most probably for isoforms devoid of a signal peptide. . |
| Tissue Expression | Broadly expressed with higher expression in heart. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Lysosome;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway |
| Research Areas | |
| Function | Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH . Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation . Has a higher catalytic efficiency towards C12-ceramides versus other ceramides . Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine . For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used . Has also an N-acylethanolamine hydrolase activity . By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes . Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis .; [Isoform 2]: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity. |
Please login to post questions
Related products
