Price range: $268.00 through $328.00
Background:
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | ATG10 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-200; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthetic peptide from human protein at AA range: 1-50 |
| Specificity | The antibody detects endogenous ATG10 |
| Gene Name | ATG10 APG10L PP12616 |
| Protein Name | Ubiquitin-like-conjugating enzyme ATG10 (Autophagy-related protein 10) (APG10-like) |
| Other Name | Ubiquitin-like-conjugating enzyme ATG10; / Autophagy-related protein 10; / APG10-like |
| Species | Human |
| Gene ID | 83734 |
| Uniprot | Q9H0Y0, |
| Species.1 | Mouse |
| Gene ID.1 | 66795 |
| Uniprot.1 | Q8R1P4 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . |
| Tissue Expression | Placenta,Testis,Thymus,Uterus, |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal |
| Research Areas | >>Autophagy - other; / >>Autophagy - animal |
| Function | Function:Plays a role in autophagy (By similarity). ATG12-conjugating enzyme (E2-like enzyme), likely serves as an ATG5-recognition molecule.,similarity:Belongs to the ATG10 family.,subunit:Interacts with MAP1LC3A. By interacting with MAP1LC3A, it plays a role in the conjugation of ATG12 to ATG5. Also able to directly interact either with ATG5 or ATG7., |
