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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® ATG3 Rabbit mAb
SKU: WR4238 Categories: ,

U-Blot® ATG3 Rabbit mAb

Western blot analysis of lysates from Hela cells, using PP2A α/β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
WR4238 Western blot analysis of lysates from Hela cells, using PP2A α/β Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

SKU: WR4238-50
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Product details

Background:This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013],

Specifications

Size50μL/100μL
TargetATG3
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)36kD
MW(Observed)36kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM0538R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameATG3 APG3 APG3L
Protein NameATG3
Other NamePP2A A; / PP2A alpha; / PP2A B; / PP2A beta; / PP2A-alpha; / PP2A-beta; / PP2AA_HUMAN; / PP2Aalpha; / PP2AB_HUMAN; / PP2Abeta; / PP2Ac; / PP2CA; / PP2Calpha; / PP2CB; / PPP2CA; / PPP2CB; / Protein phosphatase 2 catalytic subunit alpha isoform; / Protein phosphatase 2 catalytic subunit beta isoform; / Protein phosphatase type 2A catalytic subunit; / Replication protein C; / RP C; / RP-C; / RPC; / Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform; / Serine/threonine protein phosphatase 2A catalytic subunit beta isoform; / Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
SpeciesHuman
Gene ID5515;5516
UniprotQ9NT62,
Species.1Mouse
Gene ID.167841
Uniprot.1Q9CPX6,
Species.2Rat
Gene ID.224672;24673
Uniprot.2P63331;P62716
Cellular LocalizationCytoplasm
Tissue ExpressionFibroblast,Liver,Lung,Placenta,Testis,Uterus,baseline
Signaling_pathwayCellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Organismal Systems >> Nervous system >> Dopaminergic synapse;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas>>mRNA surveillance pathway; / >>Sphingolipid signaling pathway; / >>Oocyte meiosis; / >>Autophagy - other; / >>Autophagy - animal; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Adrenergic signaling in cardiomyocytes; / >>TGF-beta signaling pathway; / >>Hippo signaling pathway; / >>Tight junction; / >>Dopaminergic synapse; / >>Long-term depression; / >>Chagas disease; / >>Hepatitis C; / >>Human papillomavirus infection
FunctionFunction:GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy.,similarity:Belongs to the ATG3 family.,subunit:Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.,tissue specificity:Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.,
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